{Luciferase activity assay}{Luciferase activity assay}{Luciferase activity assay}
{Luciferase activity assay}{Luciferase activity assay}

There is no information of the target gene sequence in NCBI.
The mutation of the first target site can highly downregulate the miRNA's repression function.
The target species is virus and there are no information about it's sequence in the paper or in NCBI.
The target site sequence in the alignment can not be mapped to any candidate gene sequence in NCBI.
There is no information of the target gene in NCBI.
The target gene's accession number is from the paper and can't get the sequence of this accession.
The miRNA sequence in the alignment is not consistent with the sequence in miRBase(the first base is different.
The siRNA mimics is mixture of miR-10a and miR-10b.
The siRNA mimics is mixture of miR-10 a and miR-10b.
These findings suggest that both of the predicted target sites in TRIM71 are targets of let-7 and there are no other target sites in TRIM71.
The miRNA sequence in the second alignment is inconsistent with the sequence stored in miRBase.
Mutation of the 6th and 7th site shows that they are sufficient to regulation the expression of the target gene.
The sequence of the target site can not match the sequence of the targe gene stored in NCBI.
Double mutation of the target sites rescue the repression fact of the miRNA.
{The sequence in the alighment can not match to any potential accession number received in NCBI.}